Unchained Labs Big Tuna Automated Buffer Exchange Solution

**Preowned, no know issues or errors. Buyer can expect a working unit. Comes with a 30 day warranty. See pictures for details. FOB: FRANKLIN, MA**

The Unchained Labs Big Tuna is an automated buffer exchange solution designed for use in biopharmaceutical and life science research. It's used to perform buffer exchange and sample concentration tasks in a high-throughput and automated manner.

The Big Tuna is typically used in applications where it's essential to purify, concentrate, and exchange buffers for biological samples, such as proteins or nucleic acids, in a laboratory setting. It helps researchers save time and effort by automating these processes, increasing throughput, and ensuring consistent results.

Cut loose from manual buffer exchange

Buffer exchange and sample concentration are the time consuming, hands-on chores you have to deal with before all the things you really want to do. Big Tuna automates exchange to formulate, concentrate and clean up proteins or gene therapy vectors like AAVs, LNPs, and VLPs — with just 30 mins of setup time. Skip the slow and manual ways of prepping samples to free yourself up for other critical work.

AAV

LNP

VLP

mRNA & DNA

Protein

Go big or keep it small
Big Tuna serves up two different plate-based formats that let you exchange and concentrate 24 or 96 samples in parallel. Use Unfilter 96 to exchange as little as 100 µL for up to 96 different samples in a run. Unfilter 24 lets you go big and exchange as much as 8 mL on up to 24 samples. With different molecular weight cutoff options, you always have the right Unfilter for the job.

Hand it off
Big Tuna keeps buffer exchange even across the plate with its unique pressure-based UF/DF process and gentle mixing. Its acoustic sensor measures the volume in every well before and after each cycle to track every sample’s exchange rate. Then Big Tuna figures out on the fly how much buffer to add to even things out.

Get concentrated
Dilute samples are a giant pain and hard to handle. Big Tuna can concentrate up to 24 samples from 48 mL down to 8 mL — freeing you from all the fuss. Follow up with buffer exchange to easily get your AAVs and other low-concentration, high-volume samples right where you need them to be.

Be a control freak
Buffer exchange doesn’t have to be a shot in the dark or keep you anchored to the bench. Use the software wizard to drag and drop your samples and buffers into place, then let Big Tuna guide you through all the ways you can customize the run so it’s just right for any sample type. The easy setup means you’ll have more control over your experiment with way less hands-on time

Know it all
Load up your samples knowing they’re in good hands. Big Tuna will tell you where to put everything and how much buffer you’ll need. You can check in on progress at any time and know exactly when your exchange and concentration runs will be done. Ditch babysitting your buffer exchange and go do what you really want with your time.

Application:

Buffer exchange volume range: Unfilter 24: 0.45-8 mL Unfilter 96: 100-450 μL

Formulations Unfilter: 24: Up to 24 formulations in parallel Unfilter 96: Up to 96 formulations in parallel

Sample types: Antibodies and other proteins, nucleic acids, lipid nanoparticles (LNPs), adeno-associated viruses (AAVs), virus-like particles (VLPs)

Exchange time at full volume (96%, at 10 mg/mL IgG): Unfilter 24: 4.5 hours* Unfilter 96: 3 hours*

Protein concentration (range): Up to 200 mg/mL*

Target concentration accuracy: ± 10%*

Sample recovery: ≥96%*

System:

Volume measurement: Ultrasonic sensor

Exchange pressures: 15, 30 or 60 psi

Operating temperature: Room temperature

Buffer exchange orbital mixing: Unfilter 24: Optimized at 700 rpm Unfilter 96: Optimized at 875 rpm Duty cycle programmable

Physical: 96 cm L x 81.2 cm D x 130 cm H, 220 kg (instrument) 160 cm L x 100 cm D x 198 cm H, 270 kg (instrument and table)

Electrical Voltage: 100-260 VAC, 50-60 Hz

Nitrogen or CDA requirement: Pressure 0.55–0.9 MPa (80–130 psi) Flow rate 0.25 L/s (0.5 cfm) minimum

Consumables:

Unfilter 24: 24-well plate, up to 8 mL per well 10, 30, 100 kDa, regenerated cellulose

Unfilter 96: 96-well plate, up to 450 µL per well 3, 10, 30, 100 kDa, regenerated cellulose

Disposable tips: 1000 µL non-filtered, automatic re-use up to 12 times per exchange

Dispense precision: <300 µL: ≤3 µL 0.3–8 mL: ≤1%

Lab Robotics

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